Part 3 Methods
•Mycobacterium
parafortuitum was aerosolized from the outdoor air intake for 90 minutes and the air samples were collected at the 3 locations during the last 30 minutes of aerosolization
•The samples
were plated on trypic soy agar Petri
dishes and incubated for 2-3 days at 38°C
•The plates were then counted to determine the CFU concentration
•Microscope slides made with DAPI stain were used to determine the total number of bacteria (live and dead)