LIQUID MEDIA ANALYSIS
•pH (daily)
–pH meter
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•Sulfate concentration (Day 5 and 10 of cycle in step 2)
–Ionic Chromatography (IC) 
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•Calcium concentration (Day 10 of cycle in step 2)
–EDTA Titrimetric Method
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•Total Organic Carbon (TOC) (Day 10 of cycle in step 2)
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•Viable biomass (Day 0 & 10 of cycle in step 2)
–Phospholipids Analysis
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