FUTURE RECOMMENDATIONS
•Add neutralphilic sulfur-oxidizing microbes (NSOM) to
the liquid media.
–Monteny paper used NSOM and ASOM. They had 5-11% mass loss, 4% thickness loss,
and pH decreased to 1 vs. our
experimental results.
•Determine a method to separate surface precipitates
(gypsum, ettringite, etc) from the
concrete.
•Try longer corrosion liquid media period (more than 10
days before drying).
•Find a method to kill bacteria in the abiotic
controls.
–Day 10 liquid samples showed live bacteria based on
phospholipids despite biocide 1 g/L glutaraldehyde
and/or 5 g/L sodium azide addition.
–Alternative: Autoclave concrete specimens before
use.
•Better sterile techniques to avoid
cross-contamination.
–Uninoculated systems were positive for viable
biomass.
–Bacteria growth in post-tests not always as expected for
controls and Thiobacillus only systems.
–Separate rotary shakers and incubators for all
specimens.